Does the ovarian surface epithelium differentiate into primordial follicle and primary follicle precursor structures?
نویسندگان
چکیده
Amaç: Ovaryum yüzey epiteli hücrelerinin farklılaşma kapasitelerini hem hücre kültürü şartlarında de ovaryum doku kesitlerinde araştırmaktır.
 Gereç ve Yöntem: İki tane puberte öncesi dönemdeki (4 haftalık) dişi sıçanların ovaryumları küçük parçalara ayrılarak eksplant oluşturuldu. Miks kültüründe overyan stromal hücrelerle birlikte çoğalan izole edilerek çoğaltıldı. Bununla dokusunun histolojik incelenerek mikroskop altında görüntüleri alındı.
 Bulgular: epitelinin morfolojik görünümünün parke taşı (cobblestone) şeklinde olduğu görüldü. Faz kontrast mikroskobisi yapılan sayımda kültür kaplarında sırasıyla 2x106 3x106 hücrenin ürediği izlendi. Petri kaplarının bazı alanlarında primordial folikül benzeri yapıların oluştuğu Histolojik kesitlerde ise bazal membranın üzerinde primer öncüsü gözlemlendi.
 Sonuç: Hem kültürlerinde, oosit belirteçlerini (Gdf-9, C-Mos, Zpc, Stella) germ (Dazl,Vasa,Blimp1,Fragilis) göstermek bu hücrelerin izlememiz açısından bizlere değerli bilgiler verebilir.
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ژورنال
عنوان ژورنال: Cukurova Medical Journal
سال: 2022
ISSN: ['2602-3040', '2602-3032']
DOI: https://doi.org/10.17826/cumj.1134852